HUT CULTURE COLLECTION

HUT CULTURE COLLECTION
PROGRAM OF BIOTECHNOLOGY,GRADUATE SCHOOL OF INTEGRATED SCIENCES FOR LIFE, HIROSHIMA UNIVERSITY

Contents

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6th Catalog(PDF)
LFilamentous Fungi
LYeast
LActinobacteria
LBacteria

All Catalog (3rd)

Services
LDistribution of strains
LDeposit of strains

References
LLiterature
LMedeum
Labbreviation
LRestoration

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Procedure for reviving cultures from L-Dried materials.

Prepare the rehydration fluid and the growth medium fit in the cultures.

Score the tube around at the mid position of cotton plug inserted in the tube.

Sterilize the surface of the tube with 70% alcohol-dampened gauze. Please take care not to erase strain number written on the surface of the tube.

Wrap the tube with sterilized layered-gauze and break it gently.

Take 0.2 to 0.5 ml of rehydration fluid with a Pasteur pipette. Add a tiny drop of fluid the dried material and moisten cells well. Repeat the moistening procedure2 to 3 times. Add fluid remaining in the pipette and suspend cells well.

Spread 0.1ml of cell suspension onto agar plate medium.

Inoculate liquid medium in 2 to 3 test tubes with remaining cell suspension.

After the growth, check the purity of the culture and then use for experiments.